Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms

Journal Article · Fri Jan 31 23:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:5492052

Amann, R I; Stromley, J; Devereux, R; Key, R; Stahl, D A ^[1]

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1. Univ. of Illinois, Urbana (United States)

The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.

• OSTI ID: 5492052
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210* -- Environment
Terrestrial-- Basic Studies-- (1990-)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOASSAY
BIOREACTORS
DNA REPLICATION
DNA SEQUENCING
HYBRIDIZATION
MICROORGANISMS
MICROSCOPY
MOLECULAR BIOLOGY
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
STRUCTURAL CHEMICAL ANALYSIS
SULFATE-REDUCING BACTERIA