DNA probe arrays - accessing the genome

Conference · Mon Dec 01 04:00:00 EST 1997

OSTI ID:549014

Lipshutz, R J ^[1]

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1. Director Corporate Development, Santa Clara, CA (United States)

As the Human Genome Program and related efforts identify and determine the sequence of human genes, it is important that highly reliable and efficient mechanisms are found to access genetic information. It is only through more effective methods of mapping, monitoring expression, and screening for polymorphisms that the true benefit of the Human Genome Program will be realized. Light-directed chemical synthesis has been used to generate miniaturized, high-density arrays of oligonucleotide probes. Application-specific oligonucleotide probe array designs have been developed for polymorphisms screening, genotyping, and monitoring expression. These probe arrays are then used for parallel DNA hybridization analysis, directly yielding sequence, expression, and genotype information. Dedicated instrumentation and software has been developed for array hybridization, fluorescent detection, and data acquisition and analysis. Experiments demonstrating the effectiveness of these methods will be described. This technology will yield unprecedented amounts of genetic information challenging computational biologists to develop new methods for analysis and visualization.

• Research Organization: Association for Computing Machinery, New York, NY (United States); Sloan (Alfred P.) Foundation, New York, NY (United States)
• OSTI ID: 549014
• Report Number(s): CONF-970137--
• Country of Publication: United States
• Language: English

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
DATA ACQUISITION
DESIGN
DETECTION
DNA HYBRIDIZATION
DNA SEQUENCING
GENE MUTATIONS
GENE REGULATION
GENES
GENETIC MAPPING
GENOTYPE
MOLECULAR BIOLOGY
OLIGONUCLEOTIDES
PROBES
SCREENING
TECHNOLOGY ASSESSMENT