Mass spectrometric measurements of norepinephrine synthesis in man from infusion of stable isotope-labelled L-threo-3,4-dihydroxyphenylserine
The kinetics of stable isotope-labelled L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS), an immediate precursor of (-)-norepinephrine, was studied to investigate the pharmacologic mechanism of its therapeutic effect on orthostatic hypotension in familial amyloid polyneuropathy (FAP) and on akinesia and freezing in parkinsonism. (/sup 13/C,D)-L-threo-DOPS was synthesized, and 100 mg of the compound was infused for 2 h into two normal subjects, two FAP patients and two patients with the degenerative diseases of the central nervous system. Labelled and endogenous norepinephrine in urine and plasma was assayed simultaneously by gas chromatography/mass spectrometry. The results indicate that the increase in norepinephrine in biological fluids after administration of L-threo-DOPS is attributable mostly to norepinephrine derived from L-threo-DOPS, not to pre-formed endogenous norepinephrine released by L-threo-DOPS.
- Research Organization:
- Osaka Univ. Hospital, Japan
- OSTI ID:
- 5485575
- Journal Information:
- Life Sci.; (United States), Vol. 36:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NORADRENALINE
BIOSYNTHESIS
SERINE
METABOLISM
BIOCHEMICAL REACTION KINETICS
BLOOD PLASMA
CARBON 13
GAS CHROMATOGRAPHY
LABELLED COMPOUNDS
MASS SPECTROSCOPY
NERVOUS SYSTEM DISEASES
PATIENTS
TRACER TECHNIQUES
URINE
ADRENAL HORMONES
AMINO ACIDS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CARBON ISOTOPES
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
DISEASES
DRUGS
EVEN-ODD NUCLEI
HORMONES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
NEUROREGULATORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
WASTES
550201* - Biochemistry- Tracer Techniques