The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs

Journal Article · Sat Feb 15 04:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.4.1403· OSTI ID:5482477

Takagaki, Yoshio; Manley, J L ^[1]; MacDonald, C C; Shenk, T ^[2]

---

1. Columbia Univ., New York, NY (United States)
2. Princeton Univ., NJ (United States)

Cleavage stimulation factor is one of the multiple factors required for 3{prime}-end cleavage of mammalian pre-mRNAs. The authors have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that the 64-kDa subunit can be UV-cross linked to RNA in a polyadenylylation signal (AAUAAA)-dependent manner. They have now isolated cDNAs encoding the 64-kDa subunit of human cleavage stimulation factor. The 64-kDa subunit contains a ribonucleoprotein-type RNA binding domain in the N-terminal region and a repeat structure in the C-terminal region in which a pentapeptide sequence (consensus MEARA/G) is repeated 12 times and the formation of a long {alpha}-helix stabilized by salt bridges is predicted. An {approx}270-amino acid segment surrounding this repeat structure is highly enriched in proline and glycine residues ({approx}20% for each). When cloned 64-kDa subunit was expressed in Escherichia coli, an N-terminal fragment containing the RNA binding domain bound to RNAs in a polyadenylylation-signal-independent manner, suggesting that the RNA binding domain is directly involved in the binding of the 64-kDa subunit to pre-mRNAs.

• OSTI ID: 5482477
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 89:4; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
AZOLES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CODONS
CROSS-LINKING
DNA
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GLYCINE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYMERIZATION
PRIMATES
PROLINE
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION
VERTEBRATES