K-ras mutations in beryllium-induced mouse lung tumors

Technical Report · Tue Nov 01 04:00:00 EST 1994

OSTI ID:54823

Belinsky, S A; Mitchell, C E

Previous studies at ITRI have shown that single, nose-only exposure of F344/N rats to beryllium metal (Be) produced a 64% incidence of lung tumors over the lifetime of the rat. Long tumors induced by Be metal were subsequently analyzed for alterations in the K-ras and p53 genes. Mutation of the K-ras gene was both a rare (2 of 24 tumors) and late event in Be-induced carcinogenesis. In addition, no mutations were detected in exons 5 - 8 of the p53 gene. These results indicated that the mechanisms underlying the development of Be-induced lung cancer in rats did not involve gene dysfunction commonly associated with human non-small-cell lung cancer. The purpose of this study was to determine and compare the prevalence and specificity for mutation of the K-ras gene in lung tumors induced in the A/J mouse by Be to mutations in spontaneous tumors.

• Research Organization: Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM (United States). Inhalation Toxicology Research Inst.
• DOE Contract Number: AC04-76EV01013
• OSTI ID: 54823
• Report Number(s): ITRI--144; ON: DE95007526
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANIMAL CELLS
BERYLLIUM
CARCINOMAS
COMPARATIVE EVALUATIONS
GENE MUTATIONS
GENETIC EFFECTS
HUMAN POPULATIONS
INHALATION
INHALATION TOXICOLOGY RESEARCH INSTITUTE
MICE
PROGRESS REPORT
PROTEINS
RATS