Cyanide conversion to thiocyanate by 3-mercaptopyruvate sulfurtransferase (MPST)
Cyanide (CN) metabolism by MPST may detoxify CN to its less harmful product thiocyanate (SCN). In addition, MPST displays greater activity than rhodanese in extramitochondrial sites and in other organs which may play an important role in CN's toxicity. A 3% (W/V) homogenate of bovine kidney MPST was prepared. It was assayed using CN and mercaptopyruvate. The buffered (pH 9.5) suspension was incubated for 10 minutes. The reaction was stopped with 38% formaldehyde containing 20% ferric nitrate in nitric acid and measured at 460 nm. Phosphate buffer decreased the apparent rate of conversion of CN to SCN by 90% compared with glycine, borate, or bistris propane buffers. Hypotaurine and propanethiosulfate, but not taurine or thiomallic acid, increased the activity of MPST in a dose-related manner; e.g., hypotaurine, 0.1, 1 and 10 mmole, increased MPST indicates that several compounds increase the rate of formation of SCN from CN and may provide a potential new class of antidotes against the toxicity of CN.
- OSTI ID:
- 5481852
- Report Number(s):
- CONF-9004153--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 4:3; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spectrophotometric determination of uncomplexed cyanide and thiocyanate in wastewater with p-phenylenediamine
Sulfanegen sodium treatment in a rabbit model of sub-lethal cyanide toxicity
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
ANTITHYROID DRUGS
BODY
CARBONIC ACID DERIVATIVES
CATTLE
CYANIDES
DETOXIFICATION
DOMESTIC ANIMALS
DRUGS
ENZYME ACTIVITY
ENZYMES
HOMOGENATES
HORMONE ANTAGONISTS
KIDNEYS
MAMMALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RUMINANTS
SULFONIC ACIDS
TAURINE
THIOCYANATES
TRANSFERASES
VERTEBRATES