Investigations of particle transport in F344 rat lung using Hoechst 33342-labeled macrophages
Technical Report
·
OSTI ID:54795
Particles deposited in the lung are generally phagocytized by alveolar macrophages (AMs) and either cleared via the mucociliary escalator or transported into the interstitium. Once in the interstitium, particles may clear to the lung-associated lymph nodes (LALNs) through the lymphatic system. Species differences exist in the lung clearance rates for relatively insoluble particles that have been attributed to differences in the prevalence of the two pathways. In rodents, clearance via the mucociliary escalator appears to predominate, while in larger species, more particles are preferentially transported into the pulmonary interstitium and to the LALNs. In order to understand the role of the AMs in determining the fate of particles after they are phagocytized, it is important that the AMs themselves be labeled, independently of their particle labeling. The purpose of this study was to better define the mechanisms of AM-mediated particle transport within lung using AMs labeled with Hoechst 33342 and fluorescent polystyrene latex microspheres.
- Research Organization:
- Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM (United States). Inhalation Toxicology Research Inst.
- OSTI ID:
- 54795
- Report Number(s):
- ITRI--144; ON: DE95007526
- Country of Publication:
- United States
- Language:
- English
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