Relative adherence of Bacteroides species and strains to Actinomyces viscosus on saliva-coated hydroxyapatite

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Journal of Dental Research; (USA)

DOI:https://doi.org/10.1177/00220345890680090301· OSTI ID:5479451

Li, J; Ellen, R P ^[1]

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1. Univ. of Toronto, Ontario (Canada)

The study was designed to compare the adherence of several Bacteroides species to A. viscosus. Using 3H, we labeled 24 laboratory strains, including 13 Bacteroides species and 11 fresh clinical isolates of three Bacteroides species. Their adherence to A. viscosus bound to a saliva-coated mineral surface was quantified by liquid scintillation. Adherence relative to a standard strain, B. gingivalis 2561, was compared. Among the lab bacteroides, those of B. gingivalis (eight strains) were the greatest binders (mean, 80.5 {plus minus} 12.4%). Strains of other lab bacteroides bound less well (mean, 33.4 {plus minus} 6.3%). The difference in means was statistically significant (p less than 0.01). The mean for B. gingivalis strains was also significantly greater than that for strains of B. intermedius (51.7 {plus minus} 6.2%). Attachment of B. gingivalis was saturable in experiments in which either input concentration or time was the independent variable, indicating that B. gingivalis cells do not accumulate in this vitro simulation of plaque formation by binding to each other. Subculture did not seem to affect the degree of binding.

• OSTI ID: 5479451
• Journal Information: Journal of Dental Research; (USA), Vol. 68:9; ISSN 0022-0345
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
ADHESION
ACTINOMYCES
COMPARATIVE EVALUATIONS
GENETIC VARIABILITY
SCINTILLATION COUNTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BIOLOGICAL VARIABILITY
COUNTING TECHNIQUES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MICROORGANISMS
550701* - Microbiology- Tracer Techniques