Derivatization of benz(a)anthracene metabolites for detection by laser excited Shpol'skii spectrometry

Journal Article · Sun Jul 15 04:00:00 EDT 1990 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00213a023· OSTI ID:5479247

Weeks, S; Gilles, S; Dobson, R; Senne, S; D'Silva, A P ^[1]

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1. Iowa State Univ., Ames (USA)

The polar metabolites of polycyclic aromatic compounds (PACs) are of significant oncological interest. In contrast to parent PACs, isomer selective detection of polar PACs by laser excited Shpol'skii spectrometry (LESS) is severely limited by excessively broadened spectra and rapid photodegradation in n-alkane solvents. To minimize these limitations a 10-min derivatization procedure was developed to produce corresponding methoxy compounds, that exhibit the Shpol'skii effect. By use of the extraction and permethylation procedure, the selective detection of a mixture of hydroxybenz(a)anthracene isomers spiked at the low picogram level into urine and blood matrices was achieved. A detection limit for 1-hydroxybenz(a)anthracene was 0.6 part per trillion, or 12 fg (0.05 fmol) on a 20-{mu}L sample.

• OSTI ID: 5479247
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 62:14; ISSN 0003-2700; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTHRACENE
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CHEMICAL ANALYSIS
CONDENSED AROMATICS
DETECTION
HYDROCARBONS
LASER SPECTROSCOPY
MATERIALS
METABOLITES
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
QUANTITATIVE CHEMICAL ANALYSIS
SPECTROSCOPY
URINE
WASTES