Changes in ecdysteroids during embryogenesis of the blue crab, callinectes sapidus rathbun
Journal Article
·
· Dev. Biol.; (United States)
Total ecdysteroid titers (estimated by radioimmunoassay (RIA)) in embryos of the blue crab increased from approx. 6 ng 20-hydroxyecdysone equivalents/g in the immature embryo to a maximum of approx. 500 ng 20-hydroxyecdysone equivalents/g in maturing embryos; titers dropped to approx. 300 ng 20-hydroxyecydsone equivalents/g in prehatch embryos. High-pressure reverse-phase chromatography of the embryo extracts resolved five RIA-active components, ..cap alpha..-Ecdysone and the polar conjugate of 20-hydroxyecdysone were present in low quantities. The concentration of 20-hydroxyecdysone increased during embryogenesis to a maximum of approx. 160 ng/g in maturing embryos and decreased only slightly in the prehatch embryos. Two unidentified components were also detected and the changes in their concentrations were estimated. One, an apolar component (peak III), accounted for as much as 63% of the total RIA activity as the embryos matured. The estimated concentration of this component increased from 85 ng/g in early embryos to 475 ng/g in maturing embryos, then decreased by 50% in the prehatch embryos. The level of the other, more polar component (peak II) increased from 7.5 to 75 ng/g as the embryos developed. The increase in the concentration of ecdysteroids during embryogenesis indicates that crab embryos have the capacity to synthesize ecdysteroids and suggests that these hormones may have a physiological role in the embryonic development of crustaceans.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5475043
- Journal Information:
- Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 69; ISSN DEBIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ecdysone metabolism in premolt land crabs (Gecarcinus lateralis)
The biosynthesis and metabolism of ecdysteroids during the metamorphosis of Drosophila melanogaster
Role of ecdysteroids in the molting and reproductive cycles of the white shrimp, Penaeus vannamei
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Gen. Comp. Endocrinol.; (United States)
·
OSTI ID:6098067
The biosynthesis and metabolism of ecdysteroids during the metamorphosis of Drosophila melanogaster
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:7105735
Role of ecdysteroids in the molting and reproductive cycles of the white shrimp, Penaeus vannamei
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6718018