Characterization of the syngeneic SJL helper T-lymphoma cell interaction and the resulting growth factor products required for lymphoma growth

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:5473552

Rabinowitz, J L

B cell lymphomas (RCS) arise with high frequency in 9-13 month old SJL mice, probably from follicular center cells. Repeated additions of {gamma}-irradiated syngeneic lymph node cells (LN{gamma}), allow cultured RCS cells, cRCS-X, to be maintained in vitro indefinitely, while cRCS-X alone fail to grow. Isolated cytokines were assayed for effects on in vitro growth of cRCS-X. Murine (m) interleukin (IL)-5 and human B cell growth factor (hBCGF), exhibiting IL-5-like activity on murine B cells, were each found to promote both short and long term growth of cRCS-X cells. Although IL-1 or IFN-{gamma} alone had minimal growth promoting effects on cRCS-X cells, each synergized with either hBCGF or mIL-5. mIL-5 and hBCGF also induced colony formation by cRCS-X cells in agarose which was augmented by IL-1 or IFN-{gamma}. LN{gamma} synergized with BCGF in this assay, even in the presence of excess IL-1, while IL-5 did not. Anti-IL-5 inhibited the effect of LN{gamma} added alone, and the synergistic effect of LN{gamma} was abolished with anti-IFN-{gamma}. Thus, it was concluded that LN{gamma} contributed both IL-5 and IFN-{gamma}. IL-5 also caused much greater proliferation of normal follicular center B cells than of other B cells isolated from immunized mouse LN. Purified helper T cells from I-E{sup {minus}}, but not I-E{sup +} F{sub 1} hybrids of SJL respond to RCS. However, I-E responsive, V{beta}17a{sup +} T cells, were not essential for responsiveness, and T cells from I-E{sup +} transgenic mice, responded to RCS as did T cells from I-E{sup {minus}} litter mates. Therefore, I-E-like antigens are unlikely to be major T cell stimulating moieties on RCS cells.

• Research Organization: New York Univ., NY (United States)
• OSTI ID: 5473552
• Country of Publication: United States
• Language: English

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