Encapsulation of the UV-DNA repair enzyme T4 endonuclease V in liposomes and delivery to human cells

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Journal of Investigative Dermatology; (USA)

DOI:https://doi.org/10.1111/1523-1747.ep12277569· OSTI ID:5473193

Ceccoli, J; Rosales, N; Tsimis, J; Yarosh, D B ^[1]

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1. Applied Genetics Inc., Freeport, NY (USA)

T4 endonuclease V, a pyrimidine-dimer-specific DNA repair enzyme, was encapsulated in liposomes under mild conditions. The encapsulated enzyme was active, and when applied to ultraviolet (UV)-irradiated human cells in culture, the liposomes increased incision of UV-irradiated cellular DNA, enhanced DNA repair replication, and enhanced survival of UV-irradiated cells. This method is a first step in a new approach for topical application of DNA repair enzymes to human skin to prevent skin cancer.

• OSTI ID: 5473193
• Journal Information: Journal of Investigative Dermatology; (USA), Vol. 93:2; ISSN 0022-202X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ENDONUCLEASES
ENCAPSULATION
FIBROBLASTS
DNA REPAIR
CELL CULTURES
DNA
EPIDERMIS
GENETIC RADIATION EFFECTS
KERATIN
LIPOSOMES
MAN
SURVIVAL TIME
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DNA-ASE
ELECTROMAGNETIC RADIATION
ENZYMES
EPITHELIUM
ESTERASES
GENETIC EFFECTS
HYDROLASES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SCLEROPROTEINS
SKIN
SOMATIC CELLS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture