Synergism of cellulases from Trichoderma reesei in the degradation of cellulose
The action of cellobiohydrolases I and II (CBHI and CBHII) and endoglucanases I and II (EGI and EGII) purified from Trichoderma reesei was evaluated against various substrates. CBHI degraded the ..beta..-D-glucan from barley in a typical endo pattern. With cellulose substrates, the synergism between CBHI and endoglucanase I or II depended on the structural and ultrastructural features of the substrate. This effect, unrelated to endo-exo cooperation, was found with substrates of intermediate crystallinity whereas weak or no synergism was recorded with cellulose microcrystals or the soluble carboxy-methyl cellulose derivative. Synergistic degradation of cellulose was also recorded with mixtures of CBHI and CBHII. On the other hand, synergism between endoglucanases and CBHII followed the pattern expected for an endo-exo cooperation. These results presented support evidence for multiple types of cooperation between the cellulolytic enzymes. 30 references, 7 figures, 1 table.
- Research Organization:
- Scientific and Medical Univ., Grenoble, Sant-Martin-d'Heres, France
- OSTI ID:
- 5465674
- Journal Information:
- Bio/Technology; (United States), Journal Name: Bio/Technology; (United States) Vol. 3:8; ISSN BTCHD
- Country of Publication:
- United States
- Language:
- English
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CARBOHYDRATES
CELLULASE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL REACTIONS
DATA
DECOMPOSITION
DISPERSIONS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
INFORMATION
LYSIS
MIXTURES
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SUBSTRATES
SYNERGISM
TRICHODERMA
TRICHODERMA VIRIDE