Procalcitonin NH2-terminal cleavage peptide has no mitogenic effect on normal human osteoblast-like cells
- Endocrine Research Unit, Mayo Clinic, Rochester, MN (USA)
The NH2-terminal cleavage peptide of procalcitonin (N-proCT) recently was reported to be a bone cell mitogen. The authors have investigated the effect of N-proCT on the proliferation of normal human cells that have the phenotype of mature osteoblasts (hOB cells). N-proCT treatment for 24, 48, or 96 h in concentrations from 1 nM to 1 microM did not significantly increase (3H)thymidine uptake (means ranged from -19% to 38% of control, no significant differences) in hOB cells (6-10 cell strains per experiment) plated at four different densities. However, the hOB cells responded significantly to treatment with transforming growth factor {beta} (3 ng/ml), bovine insulin (300 micrograms/ml), or 30% fetal calf serum, which were included in all experiments as positive controls. The (3H)thymidine uptake data were confirmed in a direct cell count experiment tested at 96 h. Thus they data do not support the hypothesis that N-proCT is a potent mitogen for normal human osteoblasts.
- OSTI ID:
- 5464031
- Journal Information:
- Journal of Bone and Mineral Research; (United States), Vol. 6:5; ISSN 0884-0431
- Country of Publication:
- United States
- Language:
- English
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BONE CELLS
CELL PROLIFERATION
PEPTIDES
BIOLOGICAL FUNCTIONS
CALCITONIN
MAN
MITOGENS
PHENOTYPE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
POLYPEPTIDES
PRIMATES
PROTEINS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques