Molecular mechanisms of increased vascular smooth muscle contraction in SHR

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5462812

Sharma, R V; Aqel, M B; Butters, C; McEldoon, J; Bhalla, R C

The isometric tension development and /sup 45/Ca influx in response to NE and methoxamine stimulation were significantly (P < .05) increased in SHR caudal arteries as compared to WKY. Estimation of /sup 3/H-prazosin binding to the membranes isolated from caudal artery of WKY and SHR showed a single class of high affinity binding sites with Kd values: SHR, 128 +/- 14 pM; WKY, 141 +/- 19 pM and the Bmax values; SHR, 108 +/- 14 fmoles/mg protein; WKY, 113 +/- 21 fmoles/mg protein. Nifedipine inhibition of caudal artery contractions in response to NE stimulation was significantly greater (P < .05) in SHR as compared to WKY. On the other hand, there were no differences between WKY and SHR caudal artery rings either in the isometric tension development, /sup 45/Ca influx or nifedipine inhibition in response to K/sup +/-depolarization. Their results indicate that the increased vascular smooth muscle contraction in SHR in response to NE-stimulation may be due to increased Ca/sup 2 +/ influx through the receptor operated Ca/sup 2 +/ channels.

• Research Organization: Univ. of Iowa, Iowa City
• OSTI ID: 5462812
• Report Number(s): CONF-8604222-; TRN: 86-028371
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
MEMBRANE TRANSPORT
MUSCLES
CONTRACTION
AFFINITY
ARTERIES
BIOLOGICAL VARIABILITY
CALCIUM 45
MOLECULAR BIOLOGY
RATS
RECEPTORS
SYMPATHOMIMETICS
TRACER TECHNIQUES
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VASODILATORS
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ANIMALS
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BODY
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
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METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
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550201* - Biochemistry- Tracer Techniques