Effect of distortions in the deoxyribose phosphate backbone conformation of duplex oligodeoxyribonucleotide dodecamers containing GT, GG, GA, AC, and GU base-pair mismatches on sup 31 P NMR spectra
- Purdue Univ., West Lafayette, IN (USA)
The authors have previously suggested that variations in the {sup 31}P chemical shifts of individual phosphates in duplex oligonucleotides are attributable to torsional angle changes in the deoxyribose phosphate backbone. This hypothesis is now directly supported by analysis of the {sup 1}H/{sup 31}P two-dimensional J-resolved spectra of a number of mismatch dodecamer oligonucleotide duplexes including the following sequences: d(CGTGAATTCGCG), d(CGUGAATTCGCG), d(CGGGAATTCGCG), d(CGAGAATTCGCG), and d(CGCGAATTCACG). The {sup 31}P NMR signals of the dodecamer mismatch duplexes were assigned by 2D {sup 1}H/{sup 31}P pure absorption phase constant time (PAC) heteronuclear correlation spectra. From the assigned H3{prime} and H4{prime} signals, the {sup 31}P signals of the base-pair mismatch dodecamers were identified. J{sub H3{prime}-P} coupling constants for each of the phosphates of the dodecamers were obtained from {sup 1}H/{sup 31}P J-resolved selective proton flip 2D spectra. By use of a modified Karplus relationship, the C4{prime}-C3{prime}-O3{prime}-P torsional angles ({epsilon}) were obtained. J{sub H3{prime}-P} coupling constants were measured for many of the oligonucleotides as a function of temperature. The {sup 31}P chemical shifts follows the general observation that the more internally the phosphate is located within the oligonucleotide sequence, the more upfield the {sup 31}P resonance occurs. Analysis of the backbone torsional angle variations from the coupling constant analysis has provided additional information regarding the origin of these variations in {sup 31}P chemical shifts.
- OSTI ID:
- 5461824
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 29:22; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
AROMATICS
AZAARENES
AZINES
BARYONS
CHEMICAL SHIFT
CYTOSINE
DNA
ELEMENTARY PARTICLES
FERMIONS
GUANINE
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEIC ACIDS
NUCLEONS
ODD-EVEN NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
PURINES
PYRIMIDINES
RESONANCE
STABLE ISOTOPES
TEMPERATURE DEPENDENCE
THYMINE
URACILS