Pharmacological inhibition of eicosanoid synthesis and hyperalgesia in yeast-injected rat paws
Brewer's yeast caused an inflammation characterized by edema and hyperalgesia when injected into the hindpaw of a rat. These events were temporally distinct and each was associated with increases of specific arachidonic and oxygenation products. As determined by radioimmunoassay (RIA) on whole paw lipid extracts, the 5-lipoxygenase (5-LO) products, leukotrienes C/sub 4/ and D/sub 4/ and 5-hydroxyeicosatetraendic acid (5-HETE) were synthesized concurrently with the onset of edema (maximal at 15 minutes after yeast injection). The hyperalgesic phase of the inflammation (3-4 hr after yeast injection) was associated with increased tissue levels of the cyclooxygenase (CO) products, prostaglandin E/sub 2/ and thromboxane B/sub 2/ (TXB/sub 2/) as well as increases in levels of the 5-LO products, leukotriene B/sub 4/ (LTB/sub 4/) and 5-HETE. Pharmacological agents modulated the synthesis of eicosanoids and suppressed the hyperalgesic response.
- Research Organization:
- Merck Institute for Therapeutic Research, Rahway, NJ
- OSTI ID:
- 5459419
- Report Number(s):
- CONF-8604222-; TRN: 86-028524
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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ANIMAL TISSUES
INFLAMMATION
ARACHIDONIC ACID
RADIOIMMUNOASSAY
EICOSANOIC ACID
BIOSYNTHESIS
YEASTS
BIOLOGICAL EFFECTS
EDEMA
OXYGENASES
RATS
ANIMALS
BODY
CARBOXYLIC ACIDS
ENZYMES
FUNGI
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
PLANTS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SYMPTOMS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques