Pharmacological inhibition of eicosanoid synthesis and hyperalgesia in yeast-injected rat paws

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5459419

Opas, E E; Dallob, A; Herold, E; Luell, S; Humes, J L

Brewer's yeast caused an inflammation characterized by edema and hyperalgesia when injected into the hindpaw of a rat. These events were temporally distinct and each was associated with increases of specific arachidonic and oxygenation products. As determined by radioimmunoassay (RIA) on whole paw lipid extracts, the 5-lipoxygenase (5-LO) products, leukotrienes C/sub 4/ and D/sub 4/ and 5-hydroxyeicosatetraendic acid (5-HETE) were synthesized concurrently with the onset of edema (maximal at 15 minutes after yeast injection). The hyperalgesic phase of the inflammation (3-4 hr after yeast injection) was associated with increased tissue levels of the cyclooxygenase (CO) products, prostaglandin E/sub 2/ and thromboxane B/sub 2/ (TXB/sub 2/) as well as increases in levels of the 5-LO products, leukotriene B/sub 4/ (LTB/sub 4/) and 5-HETE. Pharmacological agents modulated the synthesis of eicosanoids and suppressed the hyperalgesic response.

• Research Organization: Merck Institute for Therapeutic Research, Rahway, NJ
• OSTI ID: 5459419
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
EDEMA
EICOSANOIC ACID
ENZYMES
FUNGI
IMMUNOASSAY
IMMUNOLOGY
INFLAMMATION
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PATHOLOGICAL CHANGES
PLANTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SYMPTOMS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
YEASTS