Hydronephrosis in mice exposed to TCDD-contaminated breast milk: Identification of the peak period of sensitivity and assessment of potential recovery

Technical Report · Tue Jan 01 00:00:00 EST 1991

OSTI ID:5458562

Couture-Haws, L; Harris, M W; McDonald, M M; Lockhart, A C; Birnbaum, L S

2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) is a potent inducer of hydronephrosis in mice both pre- and post-natally. To identify the critical period of susceptibility for development of TCDD-induced hydronephrosis in neonatal mice, as well as to characterize the potential for recovery from this renal lesion, dose-response and time-course studies were conducted using lactational exposure. Pregnant C57BL/6N mice were allowed natural delivery. In the dose-response phase of the investigation, mothers were administered 0, 3, 6, or 12 microgram TCDD/kg once by gavage on post natal day (pnd) 1, 4, 8, or 14, and dams and pups were sacrificed on pnd 26. In the time-course studies, dams were given a single oral dose of 0 or 9 microgram TCDD/kg on pnd 1, and mothers and litters were subsequently sacrificed on pnd 7, 13, 19, or 26. Neonatal kidneys were examined, and hydronephrotic severity was scored. The incidence and severity of hydronephrosis were significantly elevated above controls at all dose levels on pnd 26 following treatment on pnds 1 and 4, while treatment on pnd 8 or 14 was ineffective at inducing hydronephrosis.

• Research Organization: Environmental Protection Agency, Research Triangle Park, NC (United States). Health Effects Research Lab.
• OSTI ID: 5458562
• Report Number(s): PB-91-211441/XAB
• Resource Relation: Other Information: Pub. in Toxicology and Applied Pharmacology, v107 n2 p413-428 1991. Prepared in cooperation with National Inst. of Environmental Health Sciences, Research Triangle Park, NC. Sponsored by North Carolina Univ. at Chapel Hill, and Computer Sciences Corp., Research Triangle Park, NC
• Country of Publication: United States
• Language: English

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