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Effect of estradiol and progesterone on the rate of incorporation of radiolabeled arachidonate into phospholipids and triacylglycerols of the rat uterus

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5454101
;
To obtain information on the effect of estradiol(E) and progesterone(P) on the overall rate of the acylation and deacylation reactions with /sup 3/H-arachidonate (/sup 3/H-AA) in the rat uterus the authors investigated the effect of chronic treatment of ovariectomized rats with 2-200..mu..g/day E-dipropionate (EDP) and with 2 mg P alone or with the combination of these steroids on the extent and the time course of the in vitro incorporation of /sup 3/H-AA into various phospholipids (PLs) and the triacylglycerol fraction (TG). The results demonstrate that physiologic doses of E leads to a rapid equilibrium of the deacylation-acylation cycle only in the case of phosphatidyl inositol (PI) whereas at high (200..mu..g/day) dose level it exerts the same effect on phosphatidyl choline (PC), phosphatidyl ethanolamine (PE) as well as on TG. P alone has no remarkable effects in the ovariectomized rat, but it decreases markedly the incorporation of /sup 3/H-AA into PI and TG in intact animals. Furthermore, P decreases the incorporation of /sup 3/H-AA into PI of the ovariectomized rat treated with 2..mu..g/day EDP as well as attenuating the enhanced labeling rate of PC, PE and PI caused by higher doses. Time course studies provide evidence that all of these effects of P can be accounted for by its ability to decrease the rate of deacylation and, therefore, to prolong the time needed to attain equilibrium in the rates of acylation and deacylation reactions of the various lipids with arachidonate.
Research Organization:
St. Louis Univ. Medical Center, MO
OSTI ID:
5454101
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHOLINE
DRUGS
ESTERS
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
GLYCEROL
HORMONES
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
KETONES
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PREGNANES
PROGESTERONE
QUATERNARY COMPOUNDS
RATS
RODENTS
SACCHARIDES
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES