Effect of bioremediation on polycyclic aromatic hydrocarbon residues in soil
- Rutgers, The State Univ. of New Jersey, New Brunswick (USA)
Soil contamination (60 mg/g of soil) by a diesel oil (DO) spill was simulated in outdoor lysimeter units and the effect of bioremediation treatment consisting of liming, fertilization, and tilling on the persistence of polycyclic aromatic hydrocarbon (PAH) components of DO was measured. After solvent extraction from soil and class separation on silica gel, PAH components were identified and partially quantified by GC-ITD analysis. Residual mutagenicity and acute toxicity of the contaminated soil was also assessed by Ames and Microtox tests. Bioremediation treatment, while increasing the rate of total hydrocarbon degradation, had an even greater effect on PAH persistence, almost completely eliminating these compounds in 12 weeks. Without bioremediation, 12.5-32.5% of the higher molecular weight PAH were still present at 12 weeks. Mutagenicity and toxicity tests corroborated the above results. After substantial initial mutagenicity and toxicity, the contaminated soil approached the background level of uncontaminated soil after 12 weeks of bioremediation. Detoxification was complete in 20 weeks.
- OSTI ID:
- 5453601
- Journal Information:
- Environmental Science and Technology; (United States), Journal Name: Environmental Science and Technology; (United States) Vol. 24:7; ISSN ESTHA; ISSN 0013-936X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
020900 -- Petroleum-- Environmental Aspects
54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
AROMATICS
BIOTECHNOLOGY
CHROMATOGRAPHY
DETOXIFICATION
DIESEL FUELS
EXTRACTION
GAS CHROMATOGRAPHY
HYDROCARBONS
LYSIMETERS
MEASURING INSTRUMENTS
MUTAGEN SCREENING
ORGANIC COMPOUNDS
PETROLEUM PRODUCTS
POLYCYCLIC AROMATIC HYDROCARBONS
SCREENING
SEPARATION PROCESSES
SOILS
SOLVENT EXTRACTION