Perfluorochemical emulsions decrease Kupffer cell phagocytosis

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Hepatology (Baltimore); (United States)

DOI:https://doi.org/10.1002/hep.1840140127· OSTI ID:5451879

Bottalico, L A; Betensky, H T; Min, Y B; Weinstock, S B ^[1]

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1. Department of Chemistry, Barnard College, Columbia University, New York (USA)

One drawback to using perfluorochemical emulsions as blood substitutes is that perfluorochemical particles are cleared from the blood by the reticuloendothelial system, primarily liver and spleen. The authors measured the impact of two perfluorochemical emulsions on clearance of colloidal carbon (less than 1 microns) and 51Cr-sheep red blood cells (about 8 microns) by the reticuloendothelial system in vivo and in the isolated perfused liver. Male rats were injected with 2 ml/100 gm body wt of Fluosol-DA or Oxypherol-ET for 4 consecutive days. Carbon (1 ml/100 gm body wt) or sheep red blood cells (0.05 ml of 5% vol/vol/100 gm body wt) were then injected intravenously (in vivo) or added to perfusate. Samples were taken at several time points for 1 hr. In the isolated perfused liver, carbon clearance was depressed by 25% 1 day after treatment. Rates returned to control levels by 12 days in Fluosol-DA-treated rats but remained depressed by 67% in Oxypherol-ET-treated rats. Sheep red blood cell (8 microns) clearance was two to five times slower than carbon clearance and depressed by 40% in livers from Fluosol-DA rats 1 day and 12 days after treatment. Added serum did not improve phagocytosis. In vivo carbon clearance remained normal in Fluosol-DA-treated rats but decreased by 74% in Oxypherol-ET-treated rats 1 day after treatment, returning to normal by 12 days. Clearance rates were similar in control rats in vivo and in the perfused liver. They conclude that the isolated perfused liver is a good model to measure liver clearance function. Although low doses of perfluorochemical emulsions may depress Kupffer cell phagocytosis, general reticuloendothelial system function is not significantly compromised.

• OSTI ID: 5451879
• Journal Information: Hepatology (Baltimore); (United States), Journal Name: Hepatology (Baltimore); (United States) Vol. 14:1; ISSN HPTLD; ISSN 0270-9139
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SUBSTITUTES
BLOOD-PLASMA CLEARANCE
BODY
BODY FLUIDS
CARBON
CHROMIUM 51
CHROMIUM ISOTOPES
CLEARANCE
COLLOIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISPERSIONS
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EMULSIONS
ERYTHROCYTES
EVEN-ODD NUCLEI
GLANDS
HEMATOLOGIC AGENTS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MACROPHAGES
MAMMALS
MATERIALS
MICROSPHERES
NONMETALS
NUCLEI
ORGANS
PERFUSED ORGANS
PHAGOCYTES
PHAGOCYTOSIS
RADIOISOTOPES
RETICULOENDOTHELIAL SYSTEM
RUMINANTS
SHEEP
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES