Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: effect of inhibitors of H+ ATPase

Journal Article · Sun Mar 15 00:00:00 EST 1981 · J. Membr. Biol.; (United States)

DOI:https://doi.org/10.1007/BF01870818· OSTI ID:5451629

Steinmetz, P R; Husted, R F; Mueller, A; Beauwens, R

The coupling between H+ transport (JH) and anaerobic glycolysis was examined in vitro in an anaerobic preparation of turtle urinary bladder. JH was measured as the short-circuit current after Na+ transport was abolished with ouabain and by pH stat titration. The media were gassed with N2 and 1% CO2 (PO2 less than 0.5 mm Hg) and contained 10 mM glucose. Under these conditions, JH was not inhibited by 3 mM serosal (S) cyanide or by 0.1 mM mucosal (M) dinitrophenol. Control anaerobic lactate production (Jlac) of 47 bladders was plotted as a function of simultaneously measured JH. The slope of Jlac on JH was 0.58

• OSTI ID: 5451629
• Journal Information: J. Membr. Biol.; (United States), Vol. 59:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME INHIBITORS
BIOLOGICAL EFFECTS
GLYCOLYSIS
BIOCHEMICAL REACTION KINETICS
HYDROGEN IONS 1 PLUS
ATP-ASE
BINDING ENERGY
BLADDER
CELL MEMBRANES
ENZYME ACTIVITY
IN VITRO
MEASURING METHODS
METABOLISM
PH VALUE
TISSUE DISTRIBUTION
TURTLES
ACID ANHYDRASES
ANIMALS
BODY
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL REACTIONS
DECOMPOSITION
DISTRIBUTION
ENERGY
ENZYMES
HYDROGEN IONS
HYDROLASES
IONS
KINETICS
MEMBRANES
ORGANS
PHOSPHOHYDROLASES
REACTION KINETICS
REPTILES
URINARY TRACT
VERTEBRATES
550200* - Biochemistry
551000 - Physiological Systems