Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: effect of inhibitors of H+ ATPase

Journal Article · Sat Mar 14 23:00:00 EST 1981 · J. Membr. Biol.; (United States)

DOI:https://doi.org/10.1007/BF01870818· OSTI ID:5451629

Steinmetz, P R; Husted, R F; Mueller, A; Beauwens, R

The coupling between H+ transport (JH) and anaerobic glycolysis was examined in vitro in an anaerobic preparation of turtle urinary bladder. JH was measured as the short-circuit current after Na+ transport was abolished with ouabain and by pH stat titration. The media were gassed with N2 and 1% CO2 (PO2 less than 0.5 mm Hg) and contained 10 mM glucose. Under these conditions, JH was not inhibited by 3 mM serosal (S) cyanide or by 0.1 mM mucosal (M) dinitrophenol. Control anaerobic lactate production (Jlac) of 47 bladders was plotted as a function of simultaneously measured JH. The slope of Jlac on JH was 0.58

• OSTI ID: 5451629
• Journal Information: J. Membr. Biol.; (United States), Journal Name: J. Membr. Biol.; (United States) Vol. 59:1; ISSN JMBBB
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ANIMALS
ATP-ASE
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BLADDER
BODY
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
DECOMPOSITION
DISTRIBUTION
ENERGY
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLYCOLYSIS
HYDROGEN IONS
HYDROGEN IONS 1 PLUS
HYDROLASES
IN VITRO
IONS
KINETICS
MEASURING METHODS
MEMBRANES
METABOLISM
ORGANS
PH VALUE
PHOSPHOHYDROLASES
REACTION KINETICS
REPTILES
TISSUE DISTRIBUTION
TURTLES
URINARY TRACT
VERTEBRATES