Regulation of glutathione S-transferase Ya subunit gene expression: Identification of a unique xenobiotic-responsive element controlling inducible expression by planar aromatic compounds

Journal Article · Tue May 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.10.3826· OSTI ID:5451501

Rushmore, T H; King, R G; Pickett, C B ^[1]; Paulson, K E ^[2]

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1. Merck Frosst Centre for Therapeutic Research, Pointe-Claire-Dorval, Quebec (Canada)
2. Rockefeller Univ., New York, NY (USA)

The authors have identified a region in the 5{prime} flanking sequence of the glutathione S-transferase Ya subunit gene that contains a unique xenobiotic-responsive element (XRE). The regulatory region spans nucleotides {minus}722 to {minus}682 of the 5{prime} flanking sequence and is responsible for part of the basal level as well as inducible expression of the Ya subunit gene by planar aromatic compounds such as {beta}-naphthoflavone ({beta}-NF) and 3-methylcholanthrene. The DNA sequence of this region ({beta}-NF-responsive element) is distinct from the DNA sequence of the XRE found in the cytochrome P-450 IA1 gene. In addition to the region containing the {beta}-NF-responsive element, two other regulatory regions of the Ya subunit gene have been identified. The data suggest that regulation of gene expression by planar aromatic compounds can be mediated by a DNA sequence this is distinct from the XRE sequence.

• OSTI ID: 5451501
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:10; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DRUGS
ENZYMES
FLAVENOIDS
FLAVONES
GENE REGULATION
GENES
GLUTATHIONE
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SCINTILLATION COUNTING
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
XENOBIOTICS