In vitro stimulation of skeletal muscle protein synthesis by leucine and insulin

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5451376

Hatlestad, C L; Layman, D K

The objective of this study was to determine the potential of leu to stimulate skeletal muscle protein synthesis in the presence or absence of insulin or insulin plus a mixture of the other plasma amino acids. Male Sprague-Dawley rats weighing 75 g were fasted for 24 hrs, sacrificed, and the soleus muscle isolated from the hind limb. Protein synthesis was determined in isolated muscles incubated for 2 hrs at 37/sup 0/C in a Krebs-Ringer bicarbonate buffer containing 0.5 ..mu..Ci of /sup 14/C-tyrosine and supplemented with either 10 mM glucose (G), glucose plus 0.1 IU/m. insulin (I), or glucose, insulin and plasma levels of amino acids (IAA). Contralateral muscles were incubated in the respective media with the addition of 0.5 mM L-leucine. Insulin stimulated protein synthesis by 51% above the G group, and IAA produced a 110% stimulation. Leu stimulated protein synthesis by an additional 15-20% in each of the medias. These results confirm the ability of leu to stimulate skeletal muscle protein synthesis in vitro and establish that the effect is additive with stimulations by insulin and other amino acids.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 5451376
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
GLUCOSE
HEXOSES
HORMONES
IN VITRO
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MONOSACCHARIDES
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RATS
RODENTS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES