Influence of cytochalasin D-induced changes in cell shape on proteoglycan synthesis by cultured articular chondrocytes

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90391-6· OSTI ID:5450957

Newman, P; Watt, F M ^[1]

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1. Imperial Cancer Research Fund, London (England)

There is growing evidence that cell shape regulates both proliferation and differentiated gene expression in a variety of cell types. The authors have explored the relationship between the morphology of articular chondrocytes in culture and the amount and type of proteoglycan they synthesize, using cytochalasin D to induce reversible cell rounding. When chondrocytes were prevented from spreading or when spread cells were induced to round up, {sup 35}SO{sub 4} incorporation into proteoglycan was stimulated. Incorporation into the cell layer was stimulated more than into the medium. When the cells were allowed to respread by removing cytochalasin D, proteoglycan synthesis returned to control levels. Cytochalasin D-induced stimulation of {sup 35}SO{sub 4} incorporation reflected an increase in core protein synthesis rather than lengthening of glycosaminoglycan chains, because ({sup 3}H)serine incorporation into core protein was also stimulated. Cytochalasm D-treatment of cells in suspension caused no further stimulation of {sup 35}SO{sub 4} incorporation, suggesting that the observed effects were due to cell rounding rather than exposure to cytochalasin D per se.

• OSTI ID: 5450957
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 178:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CARTILAGE
CELL CULTURES
CELL DIFFERENTIATION
CELL PROLIFERATION
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
ENZYME INHIBITORS
EVEN-ODD NUCLEI
GLYCOPROTEINS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
SERINE
SHAPE
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
TRITIUM COMPOUNDS