Role of coronary endothelium in cyclic AMP formation by the heart

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5450879

Kroll, K; Schrader, J

In order to quantify the activation of adenylate cyclase of the coronary endothelium in vivo, endothelial adenine nucleotides of isolated guinea pig hearts were selectively pre-labeled by intracoronary infusion of tritiated (H3)-adenosine, and the coronary efflux of H3-cAMP was measured. The adenosine receptor agonist, NECA (12 ..mu..M), increased total cAMP release 4 fold, and raised H3-cAMP release 22 fold. Several classes of coronary vasodilators (adenosine, L-PIA, D-PIA, the beta 2-adrenergic agonist procaterol, and PGE1) caused dose-dependent increases in endothelial-derived H3-cAMP release. These increases were accompanied by decreases in vascular resistance, at agonist doses without positive intropic effects. Hypoxic perfusion also raised H3-cAMP release, and this was antagonized by theophylline. It is concluded: (1) cyclic AMP formation by coronary endothelium can dominate total cAMP production by the heart; (2) coronary endothelial adenylate cyclase-coupled receptors for adenosine (A2), catecholamines (beta2) and prostaglandins are activated in parallel with coronary vasodilation; (3) endothelial adenylate cyclase can be activated by endogenous adenosine.

• Research Organization: Univ. of Duesseldorf, West Germany
• OSTI ID: 5450879
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AMINES
AMP
ANIMAL TISSUES
ANIMALS
ANOXIA
AROMATICS
ARTERIES
AZAARENES
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATECHOLAMINES
CORONARIES
CYCLASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENDOTHELIUM
ENZYME ACTIVITY
ENZYMES
GUINEA PIGS
HEART
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VIVO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
MAMMALS
MEMBRANE PROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROSTAGLANDINS
PROTEINS
PURINES
REACTION KINETICS
RECEPTORS
RIBOSIDES
RODENTS
SECRETION
THEOPHYLLINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASODILATORS
VERTEBRATES
XANTHINES