A cometabolic biotransformation model for halogenated aliphatic compounds exhibiting product toxicity
- Univ. of California, Berkeley (United States)
- Stanford Univ., CA (United States)
A model is proposed to describe the rate and extent of cometabolic transformation of halogenated aliphatic compounds by resting microbial cells. The finite transformation capacity ({Tc}) of resting cells, which appears to be associated with cometabolic oxidation of many halogenated aliphatic compounds, is used to incorporate the effects of product toxicity and reductant supply into a modified expression of Monod kinetics. Applicability of the model of trichloroethylene transformation by resting cells from a mixed methanotrophic culture is evaluated by comparison with experimental data from batch transformation studies conducted over a range of conditions. A visually good and statistically reasonable fit was obtained between the experimental data and model predictions both with cells alone and with formate added as an exogenous reductant source. A comparison of parameter estimates (k and K{sub s}) derived by use of the cometabolic transformation model and those derived by use of conventional linearized Monod techniques (Lineweaver-Burk and concentration-normalized equations) indicates that, for reactions involving a finite transformation capacity, the linearized Monod equations yield artificially elevated parameters estimates.
- OSTI ID:
- 5449279
- Journal Information:
- Environmental Science and Technology; (United States), Journal Name: Environmental Science and Technology; (United States) Vol. 25:8; ISSN ESTHA; ISSN 0013-936X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DATA
DECOMPOSITION
EXPERIMENTAL DATA
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROGEN COMPOUNDS
INFORMATION
KINETICS
LIQUID WASTES
MATHEMATICAL MODELS
METABOLISM
METHANOTROPHIC BACTERIA
MICROORGANISMS
NUMERICAL DATA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
REACTION KINETICS
TOXICITY
WASTE WATER
WASTES
WATER
WATER TREATMENT