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Arachidonic acid assimilation by thrombocytes from white carneau pigeons

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5448766
;
The metabolism of arachidonic acid was investigated using thrombocyte-enriched-plasma from RBWC and WC-II white carneau pigeons, which differ genetically in their susceptibility to atherosclerosis. Thrombocytes were incubated at 42 C with (/sup 14/C) arachidonate in Puck's solution. After a 1 hour labeling period the WC-II cells had taken up 69% and RBWC 77% of the (/sup 14/C)arachidonate from the medium. When 8,11,14-eicosatrienoic acid or 5,8,11,14,17-eicosapentaenoic acid were added to incubation media the (/sup 14/C) uptake was reduced in each type cell, with WC-II exhibiting the greatest effect. Release of (/sup 14/C)molecules from cells labeled with (/sup 14/)Carachidonate was studied using calcium ionophore and indomethacin. Indomethacin inhibited (/sup 14/C) molecule release similarly in both RBWC and WC-II cells. Calcium ionophore was twice as effective in stimulating (/sup 14/C)molecule release from WC-II than RBWC cells. Therefore, the WE-II cells (from pigeons greater in susceptibility to atherosclerosis) are more sensitive to calcium ionophore than the REWC cells.
Research Organization:
Morehead State Univ., KY
OSTI ID:
5448766
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
ARACHIDONIC ACID
ARTERIOSCLEROSIS
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BIRDS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CALCIUM
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
DISEASES
ELEMENTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MATERIALS
METABOLISM
METALS
MOLECULES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGEONS
SENSITIVITY
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES