Increase in intracellular calcium and activation of protein kinase C in the expression of lymphokine receptors and the proliferation of murine B-cells
BCGF dependent B cell proliferation requires costimulation by anti-..mu.. whereas IL2 dependent B cell proliferation requires preactivation by both anti-..mu.. and LPS. These studies show that in the BCGF dependent pathway the activation signal delivered by anti-..mu.. can be mimicked by an increase in intracellular calcium ((Ca/sup 2 +/)i) induced by the calcium ionophore, Ionomycin. In the IL2 dependent pathway, the additional signal delivered by LPS that leads to IL2 receptors expression and cell proliferation, can be replaced by the phorbol ester PMA as well as the diacylglycerol sn-1,2-dioctanoyl glycerol (DiC8), both shown to induce protein kinase C (PKc) activation. In agreement with these observations they show that LPS activates PKc with no concomitant increase in intracellular calcium ((Ca/sup 2 +/)i), while anti-..mu.. induces an increase in (Ca/sup 2 +/)i but no PKc activation. (Ca/sup 2 +/)i increase and PKc activation are both critical for the expression of IL2 receptor and the subsequent IL2 proliferation whereas an increase in (Ca/sup 2 +/)i is sufficient to induce BCGF dependent proliferation. These results suggest that the activation signals leading to BCGF and IL2 dependent proliferation proceed through 2 different biochemical pathways.
- Research Organization:
- NIH, Baltimore, MD
- OSTI ID:
- 5447413
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of hydrogen sulfide on the proliferation of vascular smooth muscle cells involved in the modulation of calcium sensing receptor in high homocysteine
Neurotensin-induced Erk1/2 phosphorylation and growth of human colonic cancer cells are independent from growth factors receptors activation
Related Subjects
ANIMAL CELLS
CELL PROLIFERATION
GLYCEROL
BIOLOGICAL EFFECTS
LYMPHOKINES
RECEPTORS
PHORBOL ESTERS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
BIOLOGICAL PATHWAYS
CALCIUM
MICE
ALCOHOLS
ALKALINE EARTH METALS
ANIMALS
CARCINOGENS
ELEMENTS
ENZYMES
ESTERS
GROWTH FACTORS
HYDROXY COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
METALS
MITOGENS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)