Increase in intracellular calcium and activation of protein kinase C in the expression of lymphokine receptors and the proliferation of murine B-cells

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5447413

Proust, J; Filburn, C; Chrest, F; Buchholz, M; Nordin, A

BCGF dependent B cell proliferation requires costimulation by anti-..mu.. whereas IL2 dependent B cell proliferation requires preactivation by both anti-..mu.. and LPS. These studies show that in the BCGF dependent pathway the activation signal delivered by anti-..mu.. can be mimicked by an increase in intracellular calcium ((Ca/sup 2 +/)i) induced by the calcium ionophore, Ionomycin. In the IL2 dependent pathway, the additional signal delivered by LPS that leads to IL2 receptors expression and cell proliferation, can be replaced by the phorbol ester PMA as well as the diacylglycerol sn-1,2-dioctanoyl glycerol (DiC8), both shown to induce protein kinase C (PKc) activation. In agreement with these observations they show that LPS activates PKc with no concomitant increase in intracellular calcium ((Ca/sup 2 +/)i), while anti-..mu.. induces an increase in (Ca/sup 2 +/)i but no PKc activation. (Ca/sup 2 +/)i increase and PKc activation are both critical for the expression of IL2 receptor and the subsequent IL2 proliferation whereas an increase in (Ca/sup 2 +/)i is sufficient to induce BCGF dependent proliferation. These results suggest that the activation signals leading to BCGF and IL2 dependent proliferation proceed through 2 different biochemical pathways.

• Research Organization: NIH, Baltimore, MD
• OSTI ID: 5447413
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CALCIUM
CARCINOGENS
CELL PROLIFERATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLYCEROL
GROWTH FACTORS
HYDROXY COMPOUNDS
LYMPHOKINES
MAMMALS
MEMBRANE PROTEINS
METALS
MICE
MITOGENS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RECEPTORS
RODENTS
TRANSFERASES
VERTEBRATES