Chronic ethanol inhibits receptor-stimulated phosphoinositide hydrolysis in rat liver slices

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Alcohol; (United States)

DOI:https://doi.org/10.1016/0741-8329(91)91321-R· OSTI ID:5447412

Gonzales, R A; Crews, F T ^[1]

---

1. Department of Pharmacology, University of Texas, Austin (USA)

The effects of chronic ethanol feeding on norepinephrine (NE)- and arginine-vasopressin (AVP)-stimulated phosphoinositide (PI) hydrolysis in rat liver slices was determined. The maximum NE-stimulated PI response was significantly reduced by 40% in liver slices from 8-month-old rats which had been treated for 5 months with a liquid diet containing ethanol compared to pair-fed controls. The maximum AVP-stimulated PI response was decreased by 39% in liver slices from the ethanol-fed rats compared to control. EC50 values for NE- and AVP-stimulated PI hydrolysis in liver slices were not affected by the chronic ethanol treatment. Similar reductions in the maximal NE- and AVP-stimulated PI hydrolysis (28% and 27%, respectively) were found in 22-month-old rats which had been maintained on an ethanol containing diet for 5 months compared to pair-fed controls. The binding of (3H)prazosin and (3H)AVP to liver plasma membranes from 8-month-old ethanol-fed rats was not significantly different from binding to liver membranes from sucrose-fed controls. Our data suggest that chronic ethanol ingestion may lead to a reduction in PI-linked signal transduction in liver.

• OSTI ID: 5447412
• Journal Information: Alcohol; (United States), Vol. 8:2; ISSN 0741-8329
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ETHANOL
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
METABOLISM
CHRONIC EXPOSURE
HYDROLYSIS
IN VITRO
LIVER
NORADRENALINE
RADIOASSAY
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALCOHOLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
DECOMPOSITION
DIGESTIVE SYSTEM
DRUGS
ESTERS
GLANDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LIPIDS
LYSIS
MAMMALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
RODENTS
SOLVOLYSIS
SYMPATHOMIMETICS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques