Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Preferential location of bulged guanosine internal to a G x C tract by /sup 1/H NMR

Journal Article · · Biochemistry; (United States)
OSTI ID:5444529

A series of double-helical oligodeoxyribonucleotides of sequence corresponding to a frame-shift mutational hot spot in the lambda C/sub I/ gene, 5'-dGATGGGGCAG, are compared by proton magnetic resonance spectroscopy at 500 MHz of the exchangeable protons. Duplexes containing an extra guanine in a run of two, three, and four G x C base pairs are compared to regular helices of the same sequence and to another sequence containing an isolated bulged G, 5'-dGATGGGCAG x dCTGCGCCATC. The imino proton resonances are assigned by one-dimensional nuclear Overhauser effect spectroscopy. Resonances assigned to the G tract in bulge-containing duplexes are shifted anomalously upfield and are very broad. Imino proton lifetimes are determined by T/sub 1/ inversion-recovery experiments. The exchange rates of G-tract imino protons in bulged duplexes are rapid compared to those in regular helices and are discussed in terms of the apparent rate of solvent exchange for the isolated G bulge. Delocalization of a bulged guanosine in homopolymeric sequences can explain the observe changes in chemical shift and relaxation times across the entire G x C run, and the chemical shifts can be fit by a simple model of fast exchange between base-paired and unpaired states for the imino protons. This allows us to calculate the relative occupancies of each bulge site. In these sequences, the authors find the extra base prefers positions internal to the G tract over those at the edge.

Research Organization:
Yale Univ., New Haven, CT
OSTI ID:
5444529
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:1; ISSN BICHA
Country of Publication:
United States
Language:
English