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Human hybrid hybridoma

Journal Article · · J. Immunol.; (United States)
OSTI ID:5443955
; ; ;

Hybrid hybridomas are obtained by fusion of two cells, each producing its own antibody. Several authors have reported the construction of murine hybrid hybridomas with the aim to obtain bispecific monoclonal antibodies. The authors have investigated, in a model system, the feasibility of constructing a human hybrid hybridoma. They fused two monoclonal cell lines: an ouabain-sensitive and azaserine/hypoxanthine-resistant Epstein-Barr virus-transformed human cell line that produces an IgG1kappa antibody directed against tetanus toxiod and an azaserine/hypoxanthine-sensitive and ouabain-resistant human-mouse xenohybrid cell line that produces a human IgG1lambda antibody directed against hepatitis-B surface antigen. Hybrid hybridoma cells were selected in culture medium containing azaserine/hypoxanthine and ouabain. The hybrid nature of the secreted antibodies was analyzed by means of two antigen-specific immunoassay. The results show that it is possible, with the combined use of transformation and xenohybridization techniques, to construct human hybrid hybridomas that produce bispecific antibodies. Bispecific antibodies activity was measured by means of two radioimmunoassays.

Research Organization:
Univ. of Amsterdam, Netherlands
OSTI ID:
5443955
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:10; ISSN JOIMA
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
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ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
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CYTOLOGICAL TECHNIQUES
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MAMMALS
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