Human hybrid hybridoma

Journal Article · Sun Nov 15 00:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5443955

Tiebout, R F; van Boxtel-Oosterhof, F; Stricker, E A.M.; Zeijlemaker, W P

Hybrid hybridomas are obtained by fusion of two cells, each producing its own antibody. Several authors have reported the construction of murine hybrid hybridomas with the aim to obtain bispecific monoclonal antibodies. The authors have investigated, in a model system, the feasibility of constructing a human hybrid hybridoma. They fused two monoclonal cell lines: an ouabain-sensitive and azaserine/hypoxanthine-resistant Epstein-Barr virus-transformed human cell line that produces an IgG1kappa antibody directed against tetanus toxiod and an azaserine/hypoxanthine-sensitive and ouabain-resistant human-mouse xenohybrid cell line that produces a human IgG1lambda antibody directed against hepatitis-B surface antigen. Hybrid hybridoma cells were selected in culture medium containing azaserine/hypoxanthine and ouabain. The hybrid nature of the secreted antibodies was analyzed by means of two antigen-specific immunoassay. The results show that it is possible, with the combined use of transformation and xenohybridization techniques, to construct human hybrid hybridomas that produce bispecific antibodies. Bispecific antibodies activity was measured by means of two radioimmunoassays.

• Research Organization: Univ. of Amsterdam, Netherlands
• OSTI ID: 5443955
• Journal Information: J. Immunol.; (United States), Vol. 139:10
• Country of Publication: United States
• Language: English

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