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Regulation of adeno-associated virus gene expression in 293 cells: control of mRNA abundance and translation

Journal Article · · J. Virol.; (United States)
OSTI ID:5442467
;

The authors studied the effects of the adeno-associated virus (AAV) rep gene on the control of gene expression from the AAV p/sub 40/ promoter in 293 cells in the absence of an adenovirus coinfection. AAV vectors containing the chloramphenicol acetyltransferase (cat) gene were used to measure the levels of cat expression and steady-state mRNA from p/sub 40/. When the rep gene was present in cis or in trans, cat expression from p/sub 40/ was decreased 3- to 10-fold, but there was a 2- to 10-fold increase in the level of p/sub 40/ mRNA. Conversely, cat expression increased and the p/sub 40/ mRNA level decreased in the absence of the rep gene. Both wild-type and carboxyl-terminal truncated Rep proteins were capable of eliciting both effects. These data suggest two roles for the pleiotropic AAV rep gene: as a translational inhibitor and as a positive regulator of p/sub 40/ mRNA levels. They also provide additional evidence for a cis-acting negative regulatory region which decreases RNA from the AAV p/sub 5/ promoter in a fashion independent of rep.

Research Organization:
National Institute of Diabetes and Digestive and Kidney Disease, Bethesda, MD
OSTI ID:
5442467
Journal Information:
J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:1; ISSN JOVIA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON 14 COMPOUNDS
CHLORAMPHENICOL
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DRUGS
ELECTROPHORESIS
EMBRYONIC CELLS
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MAN
MESSENGER-RNA
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
VIRUSES