Measurement of local cerebral blood flow with (/sup 14/C)iodoantipyrine in the mouse

Journal Article · Mon Feb 01 00:00:00 EST 1988 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1988.16· OSTI ID:5442380

Jay, T M; Lucignani, G; Crane, A M; Jehle, J; Sokoloff, L

Local cerebral blood flow was measured in the mouse by means of the (/sup 14/C)iodoantipyrine method. This method has been previously used in the monkey, dog, cat, and rat, but its application to small mammals such as the mouse requires special attention to potential sources of error. The small size of the mouse brain requires special attention to the rapid removal and freezing of the brain to minimize effects of postmortem diffusion of tracer in the tissue. Because of the relatively low diameter/length ratios of the catheters needed for arterial sampling in small animals, substantial errors can occur in the determination of the time course of the (/sup 14/C)iodoantipyrine concentration in the arterial blood unless corrections for lag time and dead space washout in the catheter are properly applied. Local cerebral blood flow was measured in seven awake mice with appropriate care to minimize these sources of error. The values were found to vary from 48 ml/100 g/min in the corpus callosum to 198 ml/100 g/min in the inferior colliculus. The results demonstrate that the (/sup 14/C)iodoantipyrine method can be used to measure local cerebral blood flow in the mouse and that the values in that species are, in general, somewhat higher than those in the rat.

• Research Organization: National Institute of Mental Health, Bethesda, MD (USA)
• OSTI ID: 5442380
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Vol. 8:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
BLOOD FLOW
ANTIPYRINE
CARBON 14 COMPOUNDS
ERRORS
MICE
TRACER TECHNIQUES
ANIMALS
ANTIPYRETICS
AZOLES
BODY
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
PYRAZOLINES
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques