Clonal proliferation of cultured nonmalignant and malignant human breast epithelia

Journal Article · Sun Nov 01 00:00:00 EST 1981 · Cancer Res.; (United States)

OSTI ID:5440777

Smith, H S ^[1]; Lan, S; Ceriani, R; Hackett, A J; Stampfer, M R

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1. Peralta Cancer Research Inst., Oakland, CA

We have developed a method for clonal growth of human mammary epithelial cells of both nonmalignant and malignant origin. Plating efficiencies of 1 to 50% were obtained by seeding second-passage mammary epithelial cells on fibroblast feeder layers in an enriched medium composed of various hormones and growth factors, as well as conditioned media from three specific human cell lines. Single mammary epithelial cells seeded sparsely onto the fibroblasts underwent at least eight population doublings to form large, readily visible colonies. Optimal colony formation required both feeder cells and the enriched medium. Epithelial colonies containing at least 16 cells were visible 5 days postseeding, and these colonies continued to grow progressively. Plating efficiency and colony size were similar on ultraviolet-irradiated or nonirradiated fibroblasts. The number of colonies formed was proportional to the number of epithelial cells plated. The colonies were identified as epithelial by the presence of human mammary epithelial antigens.

• OSTI ID: 5440777
• Journal Information: Cancer Res.; (United States), Vol. 41
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
CLONING
CELL CULTURES
COLONY FORMATION
EPITHELIUM
EXTERNAL IRRADIATION
FIBROBLASTS
IN VITRO
MAMMARY GLANDS
NEOPLASMS
PATIENTS
ULTRAVIOLET RADIATION
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
GLANDS
IRRADIATION
ORGANS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
TISSUES
560121* - Radiation Effects on Cells- External Source- (-1987)