Cloning and characterization of DNA complementary to the canine distemper virus mRNA encoding matrix, phosphoprotein, and nucleocapsid protein
Journal Article
·
· J. Virol.; (United States)
OSTI ID:5438673
Double-stranded cDNA synthesized from total polyadenylate-containing mRNA, extracted from monkey kidney cells infected with canine distemper virus (CDV), has been cloned into the PstI site of Escherichia coli plasmid pBR322. Clones containing canine distemper virus DNA were identified by hybridization to a canine distemper virus-specific, /sup 32/P-labeled cDNA. Four specific clones containing different classes of sequences have been identified. The cloned plasmids contain inserts of 800 (clone 44-80), 960 (clone 74-16), 1700 (clone 364), and 950 (clone 40-9) base pairs. The sizes of the mRNA species complementary to these inserts are 1500, 1850, 1850 and 2500 nucleotides, respectively, as determined by the Northern technique. Three of the cloned DNA fragments were further identified as the reverse transcripts of the mRNA coding for the matrix, phosphoprotein, and nucleocapsid protein of CDV.
- Research Organization:
- Weizmann Institute of Science, Rehovot, Israel
- OSTI ID:
- 5438673
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 53:2; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550401 -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DNA-CLONING
ESCHERICHIA COLI
GENETICS
HYBRIDIZATION
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
MICROORGANISMS
MONKEYS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES
550401 -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
DNA-CLONING
ESCHERICHIA COLI
GENETICS
HYBRIDIZATION
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
MICROORGANISMS
MONKEYS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES