nifH,D,K gene organization in the cyanobacterium, Plectonema boryanum
Cyanobacteria are a diverse group of Gram-negative oxygenic photosynthetic prokaryotes with some species capable of fixing atmospheric nitrogen. Detailed studies dealing with the organization of nitrogen fixation genes have been limited to Anabaena, a filamentous, heterocystous cyanobacterium. The authors have determined the organization of nifH,D,K in Plectonema boryanum, a filamentous, nonheterocystous species that fixes nitrogen microaerophilically. It has been demonstrated that nifH,D,K genes are contiguous in cells grown under non-nitrogen fixing conditions using Anabaena nif genes as probes in Southern observed for all three nif genes as probes in southern hybridizations. A change in the pattern of hybridization was observed for all three nif genes isolated from cells grown under nitrogen fixing conditions. Restriction enzyme digestion experiments and analysis of cloned Plectonema nif genes are being used to determine the type of DNA modification and the location.
- Research Organization:
- Iowa State Univ., Ames
- OSTI ID:
- 5435849
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 80:4; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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