Size exclusion chromatography of alkali-solubilized coal
- Idaho National Engineering Lab., Idaho Falls (USA)
Recently, there has been an increasing interest in finding microorganisms that are able to degrade coal. An easy method that can be used to screen for such organisms is to grow them on water-solubilized coals and, using size exclusion chromatography (SEC), determine if resulting coals have a reduced molecular weight (MW). Unfortunately, there are many difficulties associated with the SEC of biologically treated, solubilized coal molecules. Interactions may be minimized by eluting samples in alkaline solutions containing amines or by using either high-performance liquid chromatography (HPLC) or conventional liquid chromatography (LC) SEC media with aqueous buffers containing Tween 80. Unfortunately, these studies have not eliminated the possibility of reversible binding occurring nor have they determined if solubilized coals are able to interact with growth medium components or microbial metabolites thereby altering the coal's apparent molecular size and shape. In this paper, the authors report optimal conditions for performing SEC of solubilized coal (e.g., SynChropak GPC 300 HPLC column using aqueous buffers at neutral pH's) and that few constituents present in microbial growth media affect these determinations.
- DOE Contract Number:
- AC07-76ID01570
- OSTI ID:
- 5435656
- Report Number(s):
- CONF-900802--
- Journal Information:
- Energy and Fuels; (United States), Journal Name: Energy and Fuels; (United States) Vol. 5:2; ISSN 0887-0624; ISSN ENFUE
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
010402 -- Coal
Lignite
& Peat-- Purification & Upgrading
010600* -- Coal
Lignite
& Peat-- Properties & Composition
ALKALINE HYDROLYSIS
AMINES
BIODEGRADATION
BROWN COAL
CARBONACEOUS MATERIALS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COAL
COAL LIQUIDS
DECOMPOSITION
ENERGY SOURCES
FLUIDS
FOSSIL FUELS
FUELS
GEL PERMEATION CHROMATOGRAPHY
HYDROLYSIS
LIGNITE
LIQUID COLUMN CHROMATOGRAPHY
LIQUIDS
LYSIS
MATERIALS
MICROORGANISMS
MOLECULAR WEIGHT
OPTIMIZATION
ORGANIC COMPOUNDS
PERFORMANCE
SEPARATION PROCESSES
SOLVOLYSIS