In vivo dose response relationship between physostigmine and cholinesterase activity in RBC and tissues of rats

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90402-5· OSTI ID:5434541

Somani, S M; Dube, S N ^[1]

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1. Southern Illinois Univ., Springfield (USA)

Dose response of physostigmine (Phy) was studied in rat using various doses. Rats were sacrificed 15 min after Phy administration. Blood and tissues were analyzed for ChE activity by radiometric method and Phy concentration by HPLC method. A comparison of ChE values in different tissues of rats indicated that ChE activity was highest in brain and least in diaphragm. The enzyme activity was eleven times more in brain as compared to diaphragm. Phy produced a dose-dependent inhibition of ChE in RBC, brain and diaphragm from 50 to 200 {mu}g/kg, then ChE inhibition was plateaued from 200 to 500 {mu}g/kg in these tissues. A dose related ChE inhibition was seen in heart and thigh muscle from 50 to 500 {mu}g/kg. Phy concentration increased linearly from 50 to 400 {mu}g/kg in plasma, brain, heart and thigh muscle. These results indicate that ChE inhibition is linear up to 200 {mu}g/kg in RBC, 150 {mu}g/kg in brain and 300 {mu}g/kg in heart. This linearity is not consistent in other tissues.

• OSTI ID: 5434541
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 44:25; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLESTERASES
CHOLINESTERASE
CHROMATOGRAPHY
DISTRIBUTION
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
ESTERASES
HYDROLASES
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
PARASYMPATHOMIMETICS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES