Sequence elements in the human osteocalcin gene confer basal activation and inducible response to hormonal vitamin D sub 3
- Baylor College of Medicine, Houston, TX (USA)
Osteoblast-specific expression of the bone protein osteocalcin is controlled at the transcriptional level by the steroid hormone 1{alpha},25-dihydroxyvitamin D{sub 3}. As this protein may represent a marker for bone activity in human disease, the authors examined the regulation of its expression at the molecular level by evaluating human osteocalcin gene promoter function. They describe regions within the promoter that contribute to basal expression of the gene in osteoblast-like cells in culture. Further, they define a 21-base-pair DNA element with the sequence 5{prime}-GTGACTCACCGGGTGAACGGG-3{prime}, which acts in cis to mediate 1{alpha},25-dihydroxyvitamin D{sub 3} inducibility of the osteocalcin gene. This response element bears sequence similarity with other short DNA segments, particularly those for estrogen and thyroid hormone, which act together with their respective trans-acting receptors to modulate gene transcription.
- OSTI ID:
- 5433899
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:12; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMISTRY
BONE CELLS
CARBON 14 COMPOUNDS
CHEMISTRY
CHLORAMPHENICOL
CHOLECALCIFEROL
CONNECTIVE TISSUE CELLS
DIAGNOSIS
DISEASES
DNA SEQUENCING
DRUGS
GENE REGULATION
GENE REPRESSORS
HORMONES
LABELLED COMPOUNDS
MUTAGENESIS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
SKELETAL DISEASES
SOMATIC CELLS
STEROID HORMONES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VITAMIN D
VITAMINS