Cytogenetic characterization of the CHO mutant EM9

Conference · Mon Dec 12 04:00:00 EST 1983

OSTI ID:5433507

Dillehay, L E; Thompson, L H; Carrano, A V

A remarkable property of the Chinese hamster ovary mutant EM9 is its very high baseline frequency of sister-chromatid exchange (SCE), about 110 per cell. EM9 is also hypersensitive (7-fold) to the induction of SCE by EMS. The high baseline SCE frequency in EM9 appears to be the result of a single mutational event. DNA replication in EM9 and AA8 was studied using pulse labeling and alkaline elution. After a 1 hour labeling period, the majority of the labeled DNA elutes rapidly indicating the small size of the nascent single-stranded molecules. In the absence of BradUrd substitution more than 50% of the labeled DNA is retained on the filter after a 1 hour chase.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5433507
• Report Number(s): UCRL-90106; CONF-831261-2; ON: DE84004820
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
EMS
ESTERS
FIBROBLASTS
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
METHYL METHANESULFONATE
MITOMYCIN
MUTAGENS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
SULFONIC ACID ESTERS
URACILS
X RADIATION