De novo purine nucleotide synthesis in the rat small and large intestine: effect of dietary protein and purines
Journal Article
·
· J. Pediatr. Gastroenterol. Nutr.; (United States)
This study assessed the pathway for de novo purine nucleotide synthesis in rat small intestinal and colonic mucosal cells, and determined the effects of dietary purines and protein on de novo purine nucleotide synthetic activity in the small intestine in vitro. Incubation of small intestinal mucosal scrapings with (/sup 14/C)glycine failed to show an active pathway of de novo synthesis; in contrast, the colon showed incorporation of (/sup 14/C)glycine into RNA. Rats fed a diet deficient in purines demonstrated increased incorporation of (/sup 14/C)glycine into RNA-adrenine in small intestinal mucosal cells. Measurement of glutamine-amidophosphoribosyltransferase demonstrated that, regardless of the purine content of the diet, enzyme activity in the small intestine is significantly lower than in the colon or liver. The results indicate that, in the small intestine of the rat, there is an inactive de novo pathway of purine nucleotide biosynthesis that can be stimulated when purines are omitted from the diet.
- Research Organization:
- Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge
- OSTI ID:
- 5433370
- Journal Information:
- J. Pediatr. Gastroenterol. Nutr.; (United States), Journal Name: J. Pediatr. Gastroenterol. Nutr.; (United States) Vol. 2:2; ISSN JPGND
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENYLIC ACID
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
CYTIDYLIC ACID
DIET
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
GLYCINE
GUANYLIC ACID
IN VITRO
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LARGE INTESTINE
MAMMALS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RATS
RNA
RODENTS
SMALL INTESTINE
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADENYLIC ACID
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
CYTIDYLIC ACID
DIET
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
GLYCINE
GUANYLIC ACID
IN VITRO
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LARGE INTESTINE
MAMMALS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RATS
RNA
RODENTS
SMALL INTESTINE
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES