Carbon-13 NMR of glycogen: Hydration response studied by using solids methods

Journal Article · Tue Jun 13 04:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00438a018· OSTI ID:5433013

Jackson, C L; Bryant, R G ^[1]

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1. Univ. of Rochester, NY (USA)

The carbon-13 NMR spectra of glycogen are reported by using the methods of magic-angle sample spinning and high-power proton decoupling to provide a dynamic report on the glucose monomer behavior as a function of hydration. Although the glycogen behaves as a typical polymer in the dry state, addition of water makes a significant difference in the spectral appearance. Water addition decreases the carbon spin-lattice relaxation times by 2 orders of magnitude over the range from 7% to 70% water by weight. The proton-carbon dipole-dipole coupling, which broadens the carbon spectrum and permits cross-polarization spectroscopy, is lost with increasing hydration over this range. By 60% water by weight, scalar decoupling methods are sufficient to achieve a reasonably high-resolution spectrum. Further, at this concentration, the carbon spin-lattice relaxation times are near their minimum values at a resonance frequency of 50.3 MHz, making acquisition of carbon spectra relatively insensitive to intensity distortions associated with saturation effects. Though motional averaging places the spectrum in the solution phase limit, the static spectrum shows a residual broader component that would not necessarily be detected readily by using high-resolution liquid-state experiments.

• OSTI ID: 5433013
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:12; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AQUEOUS SOLUTIONS
BARYONS
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
DISPERSIONS
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
GLYCOGEN
HADRONS
HYDRATION
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MIXTURES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLARIZATION
POLYSACCHARIDES
PROTONS
RELAXATION
RESONANCE
SACCHARIDES
SOLUTIONS
SOLVATION
SPIN-LATTICE RELAXATION
STABLE ISOTOPES
WATER