Aryl sulfate formation in sea urchins (Strongylocentrotus droebachiensis) ingesting marine algae (Fucus distichus) containing 2,6-dimethylnapthalene
The metabolism of tritiated 2,6-dimethylnapthalene (2,6-DMN) was studied in sea urchins (Strongylocentrotus droebachiensis) feeding on marine algae (Fucus distichus). The Fucus accumulated this hydrocarbon from sea water without converting it to metabolites. Most of the tritium accumulated by the sea urchins (e.g., 70.8% after 3 days) from feeding on 2,6-DMN-exposed Fucus was present in the exoskeleton (shell and spines). Moreover, after 3 days feeding, about 90% of the tritium in the total metabolite fraction of the gonads and digestive tract of the sea urchin was present as sulfate derivatives. These metabolites were identified through hydrolysis with aryl sulfatase, followed by thin-layer chromatography of the products. After 14 days of feeding, the tritium associated with the sulfate derivatives decreased in the gonads and digestive tract to 61 and 65%, respectively, of the total metabolite fraction. Hydroxy compounds from sulfatase hydrolysis were chromatographed using multiple elutions with toluene. The hydroxy isomers were separated and the R/sub f/ values were compared to those of pure reference compounds. The data indicated that 80% of the 2,6-dimethylnaphtyl sulfate contained the sulfate on the 1 and/or 3 position of the aromatic ring. Moreover, 6-methyl-2-naphthalenemethanol was not detected, which implies that sea urchins, unlike fish, metabolize alkyl-substituted aromatic hydrocarbons primarily through aromatic ring oxidations.
- Research Organization:
- Northwest and Alaska Fisheries Center, Seattle, WA
- OSTI ID:
- 5432881
- Journal Information:
- Environ. Res.; (United States), Vol. 27:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FUCUS
NAPHTHALENE
METABOLISM
SEA URCHINS
SULFATES
BIOSYNTHESIS
ARYL RADICALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL LOCALIZATION
CONDENSED AROMATICS
DIGESTIVE SYSTEM
EXOSKELETON
GONADS
HYDROXIDES
INGESTION
METABOLITES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALGAE
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BODY
ECHINODERMS
HYDROCARBONS
HYDROGEN COMPOUNDS
INTAKE
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PLANTS
RADICALS
SEAWEEDS
SKELETON
SULFUR COMPOUNDS
SYNTHESIS
550501* - Metabolism- Tracer Techniques