Interaction between mutations in the suppressor of Hairy wing and modifier of mdg4 genes of Drosophila melanogaster affecting the phenotype of gypsy-induced mutations
- Institute of Gene Biology and Engelhardt Institute of Molecular Biology, Moscow (Russian Federation)
The suppressor of Hairy-wing [su(Hw)] protein mediates the mutagenic effect of the gypsy retrotransposon by repressing the function of transcriptional enhancers located distally from the promoter with respect to the position of the su(Hw)-binding region. Mutations in a second gene, modifier of mdg4, also affect the gypsy-induced phenotype. Two major effects of the mod(mdg4){sup lu1} mutation can be distinguished: the interference with insulation by the su(Hw)-binding region and direct inhibition of gene expression that is not dependent on the su(Hw)-binding region position. The mod(mdg4){sup lu1} mutation partially suppresses ct{sup 6}, sc{sup D1} and Hw{sup 1} mutations, possibly by interfering with the insulation effect of the su(Hw)-binding region. An example of the second effect of mod(mdg4){sup lu1} is a complete inactivation of yellow expression in combination with the y{sup 2} allele. Phenotypic analyses of flies with combinations of mdg(mdg4){sup lu1} and different su(Hw) mutations, or with constructions carrying deletions of the acidic domains of the su(Hw) protein, suggest that the carboxy-terminal acidic domain is important for direct inhibition of yellow transcription in bristles, while the amino-terminal acidic domain is more essential for insulation. 31 refs., 1 fig., 6 tabs.
- OSTI ID:
- 543043
- Journal Information:
- Genetics, Journal Name: Genetics Journal Issue: 2 Vol. 142; ISSN GENTAE; ISSN 0016-6731
- Country of Publication:
- United States
- Language:
- English
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