Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila

Journal Article · Sat Jun 01 04:00:00 EDT 1996 · Genetics

OSTI ID:543019

Brickner, J H; Lynch, T J; Zeilinger, D; Orias, E ^[1]

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1. Univ. of California, Santa Barbara, CA (United States)

Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from C3-368-5 (C3 strain). Polymorphisms were mapped to chromosomes using a panel of monosomic strains constructed by crossing B strain-derived nullisomic strains to inbred strain C3. They map to all five chromosomes and appear to be evenly distributed throughout the genome. Chromosomal groups were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chromosomes 1R, 2L, 3 and 5. The RAPD method appears useful for the construction of a genetic map of the Tetrahymena genome based on DNA polymorphisms. 37 refs., 4 figs., 6 tabs.

• OSTI ID: 543019
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 2 Vol. 143; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMES
DNA
EVALUATION
GENE AMPLIFICATION
GENETIC MAPPING
GENETICS
IDENTIFICATION SYSTEMS
MEIOSIS
MUTATIONS
POLYMERASE CHAIN REACTION
STRAINS
TETRAHYMENA