Induced biosynthesis of cutaneous prostaglandins by ionizing irradiation

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-169-41362· OSTI ID:5430006

Ziboh, V A ^[1]; Mallia, C; Morhart, E; Taylor, J R

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1. Univ of California, Davis

This study has demonstrated that exposure of white domestic pig skin to a single treatment of X-ray irradiation (300-1000 rad) results in a rapid release of epidermal arachidonic acid followed by its transformation via the cyclooxygenase pathway into prostaglandin E/sub 2/ (PGE/sub 2/). Beyond 24 hr the epidermal PGE/sub 2/ decreased steadily. The time-dependent biosynthesis of epidermal PGE/sub 2/ can be blocked by the immediate topical application of 2.5% indomethacin in ethanol to the irradiated skin area. In order to seek an explanation for the progressive decrease of epidermal PGE/sub 2/ beyond 24 hr we assayed for the activity of the NADPH-dependent PGE/sub 2/-9-ketoreductase (an enzyme which catalyzes the transformation of PGE/sub 2//sub ..cap alpha../ in the 105,000/sub g/ supernatant fractions prepared from the irradiated and nonirradiated epidermal specimens. Our data revealed that the activity of this interconverting enzyme increased steadily beyond 24 hr. These results indicate that PGE/sub 2/ formed as a result of exposure of skin to X rays is metabolized into PGE/sub 2//sub ..cap alpha../. The biological significance of this interconversion and the role of PGE/sub 2//sub ..cap alpha../ in X-ray-induced injury to the skin remains to be determined.

• OSTI ID: 5430006
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 169:3; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DOMESTIC ANIMALS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
EPIDERMIS
EPITHELIUM
INHIBITION
IONIZING RADIATIONS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROSTAGLANDINS
RADIATION EFFECTS
RADIATIONS
SKIN
SWINE
SYNTHESIS
TIME DEPENDENCE
TISSUES
VERTEBRATES
X RADIATION