Sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status
Journal Article
·
· Anal. Biochem.; (United States)
Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral alimentation. This paper presents a biotin assay utilizing radiolabeled avidin in a sequential, solid-phase method; the assay has increased sensitivity compared to previous methods (greater than or equal to 10 fmol/tube), correlates with expected trends in biotin concentrations in blood and urine in a rat model of biotin deficiency, and can utilize commercially available radiolabeled avidin.
- Research Organization:
- Univ. of Iowa, Iowa City
- OSTI ID:
- 5429617
- Journal Information:
- Anal. Biochem.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BIOTIN
RADIOASSAY
BIOLOGICAL MODELS
BLOOD PLASMA
RADIOISOTOPES
RATS
TRACER TECHNIQUES
URINE
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CARBOXYLIC ACIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RODENTS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WASTES
550201* - Biochemistry- Tracer Techniques
BIOTIN
RADIOASSAY
BIOLOGICAL MODELS
BLOOD PLASMA
RADIOISOTOPES
RATS
TRACER TECHNIQUES
URINE
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
CARBOXYLIC ACIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RODENTS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WASTES
550201* - Biochemistry- Tracer Techniques