Specific and stable labeling of antibodies with technetium-99m with a diamide dithiolate chelating agent

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.11.4025· OSTI ID:5420523

Fritzberg, A R; Abrams, P G; Beaumier, P L; Kasina, S; Morgan, A C; Rao, T N; Reno, J M; Sanderson, J A; Srinivasan, A; Wilbur, D S; Vanderheyden, J L ^[1]

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1. NeoRx Corporation, Seattle, WA (USA)

Technetium-99m labeling of antibodies has been suboptimal because of low affinity adventitious binding, nonspecific labeling, and loss of immunoreactivity. The diamide dithiolate ligand system (N{sub 2}S{sub 2}) forms highly stable, well-defined tetradentate complexes with Tc(V). Antibodies and their fragments have been labeled by conjugation of preformed {sup 99m}Tc-4,5-bis(thioacetamido)pentanoate active ester to protein amine groups to give a chemically known {sup 99m}Tc-N{sub 2}S{sub 2} complex covalently linked to antibody. Evaluations of the {sup 99m}Tc-N{sub 2}S{sub 2}-bound antibodies and their fragments have shown high stability and retained immunoreactivity.

• OSTI ID: 5420523
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:11; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CAMERAS
CHELATING AGENTS
CHEMICAL PREPARATION
CHROMATOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC USES
DISEASES
DRUGS
GAMMA CAMERAS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MELANOMAS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TECHNETIUM 99
TECHNETIUM ISOTOPES
USES
VERTEBRATES
YEARS LIVING RADIOISOTOPES