Flow cytometer for identifying algae by chlorophyll fluorescence
Identification of algae in water samples is carried out with a flow cytometer having at least one high intensity light source to provide a light path such as laser, means for passing the algae through the light path and a system of detectors arranged to measure chlorophyll fluorescence and at least two other parameters such as forward light scattering, perpendicular light scattering, backward light scattering, pulse length and/or shape of the scattered signals, fluorescence and pulse length and/or shape of the fluorescence signals. In order to separate the light output from the flow cytometer into components which can yield useful measurements, a system of partially reflecting mirrors and dichroic mirrors is used. Preferably, the high intensity light source is two independent lasers having parallel light paths one of which is separated in a UV range and the other in an all lines mode.
- Assignee:
- Nederlandse Centrale Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek (Netherlands)
- Patent Number(s):
- US 4500641
- OSTI ID:
- 5418519
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
47 OTHER INSTRUMENTATION
ALGAE
CARBOXYLIC ACIDS
CHEMISTRY
CHLOROPHYLL
DETECTION
FLUORESCENCE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LASERS
LIGHT SCATTERING
LUMINESCENCE
MEASURING INSTRUMENTS
MEASURING METHODS
MIRRORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
SCATTERING
WATER CHEMISTRY